PROPAGATION OF SOME TOMATO HYBRIDS VIA TISSUE CULTURE TECHNIQUE

Sherbeni, Fatma S.; El-Ghamriny, E. A.; Nawar, Dalia A.S.; Zyada, H. G.

PROPAGATION OF SOME TOMATO HYBRIDS VIA TISSUE CULTURE TECHNIQUE

Document Type : Original Article

Authors

Hort. Dept., Fac. Agric., Zagazig Univ., Egypt

Abstract

This investigation was carried out at the Tissue Culture Lab., Hort. Dept., Fac.
Agric., Zagazig Univ., Egypt during the two successive seasons of 2014 and 2015 to produce
transplants of two tomato hybrids through tissue culture technique. A protocol was developed for
shoot multiplication and rooting in two tomato (Lycopersicon esculentum Mill.) hybrids i.e., Silla 108
and Farrah. Shoot tips were cultured into Murashige and Skoog media (MS medium) with different
concentrations of benzyl adenine (BA) and Kinetin (Kin) 1.5 mg BA/l, 1.5 mg BA/l + 1 mg Kinetin/l,
3 mg Kin/l and 4 mg Kin/l) compared with MS basal medium as a control treatment, the obtained
results illustrated that supplementing BA at 1.5 mg/l gave the highest value of number of
shoots/explant of Sila 108 hybrid followed by kinetin at 4 mg/l for Farrah hybrid in both seasons.
Multiplicated shoots of two tomato hybrids were excised and cultured on MS medium supplemented
with different rooting growth regulators including indole acetic acid (IAA) at 0.5 and 1 mg/l,
Naphthalene acetic acid (NAA) at 1 mg/l and indole butyric acid (IBA) at 1mg/l compared to the
control treatment (without growth regulators). From the foregoing results, it could be concluded that
supplementing IAA at 1 mg/l and NAA at 1 mg/l to MS media were the best treatment for rooting
stage of Farrah and Sila 108 tomato hybrids. The plantlets were transferred to plastic cups containing
peatmoss + sand at 1:1, peatmoos + vermiculite at 1:1 (V/V) or peatmoss alone. It could be concluded
that the agriculture media for acclimatization of plantlets of Farrah and Sila 108 tomato hybrids
produced from tissue culture technique were peatmoss and peatmoss+ vermiculite at 1:1 (V/V).

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