CHARACTERIZATION OF TOLERATE BIOMARKER IN FABA BEAN (Vicia faba L.) AGAINST Orobanche crenata

Document Type : Original Article

Authors

1 Agric. Biochem. Branch., Soil and Water Sci. Dept., Fac. Technol. and Deve., Zagazig Univ., Egypt

2 Agric. Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt

Abstract

This study was conducted in Zagazig University, Egypt to evaluate tolerate biomarkers in faba bean against Orobanche crenata. Two faba bean genotypes were choicen, known as Msr-3 (tolerate) and Giza-716 (susceptible). Obtained results showed that tolerate genotype has high content of chlorophyll a (1.20 mg/ g fresh weight), chlorophyll b (0.69 mg/g fresh weight), total chlorophyll (1.89 mg/ g fresh weight) and carotenoid (0.97 mg/ g fresh weight). Also tolerate faba bean contained high content of componants known as major osmotically-active componants, namely total soluble sugar (0.045 mg/ml), total protein (7.85%), Na+ (39.16ppm), K+ (59.24ppm) and polar amino acids (78.54%). Tolerate faba bean also showed a high content of total phenolic compounds (348.84 ppm calculated as a Gallic acid), conjugated phenolic compounds (227.69 ppm calculated as a Gallic acid). As well as tolerate faba bean (Msr-3) has high catalase activity (0.39 mg. protein-1).

Keywords