IN VITRO REGULATION AND ENHANCEMENT OF ORGANOGENESIS IN SIX CANOLA (Brassica napus, L.) GENOTYPES

Attaya, Ahmed S.; El-Sarag, Eman I.; Mekki, B- E.; Ibrahim, Aya E-E.

doi:10.21608/zjar.2017.52912

IN VITRO REGULATION AND ENHANCEMENT OF ORGANOGENESIS IN SIX CANOLA (Brassica napus, L.) GENOTYPES

Document Type : Original Article

Authors

¹ Plant Prod. Dep., Fac. Environ. Agric. Sci., Arish Univ., North Sinai, Egypt

² Plant Prod. Dept., Fac. Environ. Agric. Sci., Arish Univ., North Sinai, Egypt

³ Field Crops Res. Dept., Nat. Res. Cent., Giza, Egypt

10.21608/zjar.2017.52912

Abstract

This study was designed to investigate the effect of some factors affecting on in vitro propagation of six canola genotypes (Serw-6, Pactol, Wan-you 25, RG-4514, Serw-4 and AD-201/Gi/ 51). During establishment stage, different medium types (MS and B5) and cytokinin types (BA, Kin and 2ip) were investigated. MS medium supplemented with BA at 1.0 mgl^-1 proved to be the best treatment for all tested genotypes during this stage. Also, different explant types (shoot tip, internode and hypocotyl), BA concentrations (0.0, 1.0, 3.0 and 5.0 mg l^-1) were examined during multiplication stage. Results indicated that while explant type had no effect on shoot induction and growth, BA proved to be the best cytokinin especially at 1.0 mgl^-1 since it produced the maximum number of shoots/ explant for the most tested genotypes. During rooting stage, effects of MS medium strength (full and half) and auxin type (IAA, IBA and NAA) at the concentration of 1.0 mgl^-1 were evaluated. Generally, using of full MS medium supplemented with 1.0 mgl^-1 IBA stated to be the most suitable treatment for root induction and growth of the most investigated genotypes. Obtained plantlets were successfully acclimatized (60-80% survivability) in peat moss and sand (1:1, V/V) medium in the greenhouse.

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