Document Type : Original Article
Authors
1
Agron. Dept., Fac. Agric., Zagazig Univ., Egypt
2
Genet. Res. Dept., Field Crops Res. Inst., ARC, Bahteem, Giza, Egypt
3
Seed Tech. Res. Dept., Field Crops Res. Inst., ARC, Giza, Egypt
Abstract
The present study was carried out at Genetic Resources Department, Bahteem,
Egypt, during 2014/2015 and 2015 /2016 seasons, to evaluate five new faba bean cultivars (Misr 3,
Sakha 4, Wadi1, Nubaria 1and Nubaria 3) as well as five old ones (Giza 2, Giza 3, Giza 40, Giza 429
and Giza 843) for earliness, yield, yield attributes and quality characteristics through mean
performance, correlation coefficient, path coefficient and molecular markers of RAPD-PCR analysis.
The experimental design was a randomized complete block design in four replications. Results
indicated highly significant differences among the ten faba bean cultivars for earliness characters (days
to first flower, days to 50% flowering and days to fruiting), yield and its attributes (plant height,
number of branches/plant, number of fruiting nodes/plant, pod length, number of pods/node, number
of pods/plant, number of seeds/pod, number of seeds/plant, 100- seed weight and seed yield/ plant) as
well as quality characters (protein and carbohydrate contents) in the two seasons. The new cultivars
were earlier than the old once during the two seasons. The new faba bean cultivar Nubaria 1 gave the
highest productivity however Sakha 4 recorded the highest value of protein and carbohydrate contents
in the two seasons. Seed yield/plant appeared positive and significant correlation with each of pod
length, number of pods/plant, number of seeds/ pod, number of seeds/plant and 100-seed weight
during the two seasons. Whereas, negative and significant correlation was detected between seed
yield/plant and each of plant height and number of fruiting nodes/plant in both seasons. Path
coefficient analysis showed that number of seeds/plant followed by 100-seed weight had the highest
direct effect on seed yield/plant during the two seasons. Five primers were used for RAPD analysis to
determine genetic variability among the ten cultivars of faba bean, and produced 32 bands, 21 of them
were polymorphic with 65.6% polymorphism. Four positive bands markers were recorded in faba bean
cultivar Giza 3 in two primes (C06 and C19), and two positive band markers in faba bean cultivar Giza
40 in the two primers (B09 and C19). While, one negative band marker in Giza 429 and one positive
marker in Giza 843 were generated by primer (C19).
Keywords
)
View on SCiNiTO