Document Type : Original Article
Authors
1
Plant Prot. Dept., Fac. Agric., Zagazig Univ., Egypt
2
Genet. Dept., Fac., Agric., Zagazig Univ., Egypt
3
Hortic. Dept., Fac. Agric., Zagazig Univ., Egypt
Abstract
This study was carried out to determine the nematicidal efficiency of seven
bionematicides (Nema end, Nema cont, Nema clean, Nema K, Bio-zeid, Bio-arc and Nemex)
compared with one nematicide (Nemacur 10%) on three cultivars of tomato against the root-knot
nematode (RKN) Meloidogyne incognita under greenhouse conditions. The screened tomato cultivars
(endless summer, supermarmand and jueblle) were significantly different in their response to nematode
infection. Detectable tolerance to nematode infection was recorded with cultivar Endless summer and
Juebelle while the least tolerance was recorded with supermarmand cultivar. Number of galls and egg
masses were significantly decreased in the investigated cultivars treated with Nemacur, and Nema k,
Nema cont and Bio-zeid (82.49, 73.97; 68.48, 50.00; 30.74, 33.34; 61.09, 40.62%), respectively in
endless summer, (78.90, 69.92; 73.70, 55.75; 68.51, 19.48; 72.31, 43.38%), respectively, in
supermarmand and (71.15, 67.19; 61.90, 40.64; 59.38, 40.64; 41.18, 15.63%) respectively, in Juebelle.
Fresh and dry weights of shoot had significantly increased with same components, (5.10, 8.77; 3.34,
7.21; 2.44, 3.24; 2.69, 4.80%) respectively, in Endless summer and (8.03, 5.09; 6.80, 3.96; 4.54, 3.75;
6.02, 2.44%) respectively, in Juebelle compared with untreated plants, while supermarmand was the
most susceptible for infection with RKN. Random amplified polymorphic DNA (RAPD) marker using
four primers detected polymorphism in DNA in percentage ranged between 33.33 – 75% with total
polymorphism 58.33%. Primer OPC-09 gave the highest polymorphism (75%) while OPB-18 gave the
lowest polymorphism (33.33%).
Keywords
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