ASSESSING ECONOMIC ANALYSIS AND MORPHOLOGICAL TRAITS OF TOMATO TISSUE CULTURE PROPAGATION

Document Type : Original Article

Authors

1 Genet. Dept., Fac. Agric., Zagazig Univ., Egypt

2 . Hort. Dept., Fac. Agric., Zagazig Univ., Egypt

10.21608/zjar.2025.451797

Abstract

In order to assess the ability of establishment of tomato tissue culture protocol and economic analysis an experiment was done at Plant Tissue Culture Laboratory, Genetic Department, Faculty of Agriculture, Zagazig University, Egypt. To produce a healthy transplants of different tomato hybrids (Ace 55 VF-Q, Marglobe IMP-VF, Earl Pearson-USA/Q and Marmand-VF), the experiment divided into four stages (seed germination, multiplication, rooting and acclimatization). Shoot number of tomato hybrids gradually increased as number of sub-culture was repeated from initiation, sub-culture one to sub-culture two. In multiplication stage, nodals about 2-3 mm from the previously achieved seedlings were cultured on MS supplemented with 1.5 mg/l of BA + 1mg/l of kinetin. This treatment significantly increased shoots number per explant, shoot length and leaf number per shoot compared to control. The highest values of these traits were noticed on Ace 55 VF-Q hybrid compared to the other hybrids under study. IAA at 1mg/l and NAA at 1mg/l in rooting stage produced the maximum roots number per plantlet and longest roots. Ace 55 VF-Q hybrid recorded the longest root and the higher number of roots per plantlet. In acclimatization stage, the highest transplants survival percentage was achieved with peat moss + vermiculite at 1: 1 (V/V). From economic analyses, the total cost of one transplanting in the tissue culture lab was 0.789 Egyptian pounds, while the total cost of one seedling in the traditional nursery in the summer season was 2.50 Egyptian pounds and in the winter season 1.25 Egyptian pounds

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Zagazig Journal of Agricultural Research
Volume 52, Issue 4
July and August 2025
Pages 849-859

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