CHEMICAL ANALYSIS, ANTIOXIDANTS AND SUGARS IN WHITE MULBERRY FRUITS

Document Type : Original Article

Authors

1 Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt

2 Med.and Aromatic Plants Res. Dept., Pharm. and Drug Indust. Res. Inst., Nat. Res. Centre, PO Box 12622, Cairo, Egypt

Abstract

The fruits of white mulberry are widespread fruit in Egypt and are eaten by most people with acceptable taste, so we tried in this research to show the chemical composition of the fruits and the effectiveness of white mulberry as an antioxidant and also know the quality of the polysaccharide that is found in whitewhite mulberryand study the properties of sugar many in terms of the quality of the structural units involved in the composition and effective groups ofits molecular weight and also its ability as an antioxidant and to achieve this purpose we have collectedthe fruits of white mulberryfromThe local market of the city of Zagazigwaswashed and dried and then grindingand storage in appropriate conditions of temperature (4°C) and the chemical composition of moisture,total ash, crude protein, crude fat, fiber and carbohydrates was estimated by the difference and the results were asfollows asa percentage of the dry sample of white mulberry fruits (12.02 - 14.42 - 21 - 4.83 - 15 - 59.75) respectively.Total phenols and total flavonoids were estimated through two types of extracts, aqueous extract and alcoholic extract to determine the effectiveness of white mulberry fruiting as an antioxidant, where the results were (23.5–13.9 mg gallic acid/gram total phenolatextract aqueous and alcoholic extract respectively As for the total flavonoids, the results were (1.43 – 2.54 mg brochuretin/gram aqueous and alcoholic extract, respectively, and finally. The antioxidant activity of white mulberry fruits was estimated by DPPH method and the results obtained were (6.66 – 1.97 mg vitamin C/gram aqueous and alcoholic extract respectively.The sugar was separated from many white mulberry fruits and purified and it became clear from the results to obtain two types of sugar many in the fruits of white mulberry differ in molecular weight and also in the type ofstructural units involved in the composition where the molecular weightsugarwas reached 1.25 x 104- 5.29 x 104 Dalton and called them fraction 1 and fraction 2 respectively The quality of monosaccharide wasglucose – Mannose - Arabenose- fructose -Mannuronic acid in fractions WMP1 or fraction WMP2 It contains the following structural units (glucose - galactose - arabinose - manuuronic acid acid) Through theresults obtained fromthe HPLC device, the quality of theeffective groups in polysaccharide fractions in white mulberry fruits was also identified through the infrared spectrum, which showed peaks appear in 1728 - 1619.2 - 3345.6 cm-1 for samples Which indicates the presence ofthe CH2 groupand the C=Ogrouphydroxyl group, respectively. Through the results obtained, we can say that the consumption of white mulberry fruits provides the human body with natural antioxidant compounds and also a high content of protein, salts and total carbohydrates.

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