EFFECT OF GIBBERELLIC ACID ON STRAWBERRY (Fragaria X ananassa Duch.) MICROPROPAGATION DURING MULTIPLICATION STAGE

Mohamed, Mohamed E.M.; Fekry, Wafaa A.; Wahdan, H. M.; Bardisi, A.

doi:10.21608/zjar.2016.101011

EFFECT OF GIBBERELLIC ACID ON STRAWBERRY (Fragaria X ananassa Duch.) MICROPROPAGATION DURING MULTIPLICATION STAGE

Document Type : Original Article

Authors

¹ Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt

² Hort. Dept., Fac. Agric., Zagazig Univ., Egypt

10.21608/zjar.2016.101011

Abstract

The present work was conducted at the tissue culture lab. of the Center of Genetic Engineering and Biotechnology, Fac. Agric., Ain Shams University, Egypt. It aims to reveal the micropropagation possibilities of Festival and Marquez strawberry cultivars using runners meristem culture on different concentrations of gibberellic acid (GA₃); i.e., 0.1, 0.2, 0.3, 0.4 and 0.5 mgl^-1) in multiplication stage. Supplementation the media with GA₃ at 0.4 mgl^-1 gave the highest values for number of shoots per explant and leaf number per shoot, whereas GA₃ at 0.5mgl^-1for shoot length was found to be the most appropriate concentration. In addition, Festival strawberry cultivar achieved the best results of all features under study than Marquez cultivar. The most favourble interaction treatment for maximizing number of shoots/explant and number of leaves/shoot was MS medium containing 0.1 mgl^-1 benzyladenine (BA) + 0.4 mgl^-1 GA₃ with Festival strawberry cultivar.

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